THE UNIVERSITY OF WESTERN AUSTRALIA
FACULTY OF MEDICINE AND DENTISTRY

Qualifications
BSc (Hons), University of WA (1986)
PhD, University of WA (1991)

Research Interests
Molecular toxicology
Protein and DNA adduction by reactive intermediates
Oxidative stress & free radical biology
Toxicology of acrolein and inhaled smoke
Toxicology of blue-green algal toxins

Professional Associations
Member, Australasian Society for Clinical & Experimental Pharmacologists & Toxicologists (ASCEPT)
Member, Royal Australian Chemical Institute (RACI)
Member, Society for Free Radical Research Australasia (SFRRA)
Member, Mutagenesis & Experimental Pathology Society of Australia (MEPSA)

Work History
1990-1993, Research Associate, Center for Molecular Toxicology, Vanderbilt University, Nashville, Tennessee
1993-2005, Lecturer/Senior Lecturer, Dept Clinical & Experimental Pharmacology, The University of Adelaide, South Australia
2000-2001, Visiting Scientist, School of Pharmacy, University of Colorado Health Science Center, Denver, Colorado

Selected Publications

Kingma, P. S., Corbett, A. H., Burcham, P. C., Marnett, L. J., and Osheroff, N. (1995) Abasic sites stimulate Double-stranded DNA cleavage Mediated by Topoisomerase II: DNA lesions as Endogenous Topoisomerase II Poisons. The Journal of Biological Chemistry , 270, 21441-21444.

Burcham, PC, & Kuhan, YT (1996) Introduction of carbonyl groups into proteins by the lipid peroxidation product, malondialdehyde. Biochemical & Biophysical Research Communications, 220, 996-1001.

Burcham, P. C., and Kuhan, Y. T. (1997) Diminished susceptibility to proteolysis after protein modification by the lipid peroxidation product malondialdehyde. Inhibitory role for crosslinked and non-crosslinked adducted proteins. Archives in Biochemistry & Biophysics, 340, 331-337.

Harkin, LA, Butler, LM, & Burcham, PC (1997) Role of G?T transversions in the mutagenicity of alkylperoxyl radicals: Induction of alkali-labile sites in phage M13mp19. Chem Res Toxicol, 10, 575-581.

Harkin, L. A., and Burcham, P. C. (1997) Formation of novel C1-oxidised abasic sites in alkylperoxyl radical-damaged plasmid DNA. Biochemical and Biophysical Research Communications, 237, 1-5.

Sallustio, B. C., Harkin, L. A., and Burcham, P. C. (1997) Genotoxicity of acyl glucuronide metabolites formed from clofibric acid and gemfibrozil: a novel role for Phase II-mediated bioactivation in the hepatocarcinogenicity of the parent aglycones? Toxicology and Applied Pharmacology, 147, 459-464.

Burcham, P. C. (1998) Genotoxic lipid peroxidation products: A review of their DNA-damaging properties and role in the formation of endogenous DNA adducts. Mutagenesis, 13, 287-305.

Burcham, P. C., and Harkin, L. A. (1999) Mutations at G:C Base-Pairs Predominate After Replication of Peroxyl Radical-Damaged pSP189 Plasmids in Human Cells. Mutagenesis, 14, 135-140.

Burcham, P. C. (1999) Internal Hazards: Baseline DNA Damage by Endogenous Products of Normal Metabolism. Mutation Research, 443, 11-36.

Nicholls-Grzemski, FA, Calder IC, Priestly, BG, and Burcham, PC (2000) Clofibrate-induced in vitro hepatoprotection against acetaminophen is not due to altered glutathione homeostasis. Toxicological Sciences, 56, 220-228.
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Nicholls-Grzemski, FA, Belling, BG, Priestly, BG, Calder, IC, and Burcham, PC (2000) Clofibrate-pretreatment in mice confers resistance against hepatic LPO. J. Molec & Biochem Toxicology 14, 335-345.

Burcham, P.C., and Fontaine, F. (2001) Extensive protein carbonylation precedes acrolein-mediated cell death in mouse hepatocytes. J. Molecular & Biochemical Toxicology 15, 309-316.

Froscio, S.M., Humpage, A.R., Burcham, P.C., and Falconer, I.R. (2001) Cell-free protein synthesis inhibition assay for the cyanobacterial toxin cylindrospermopsin. Environmental Toxicology, 16, 408-412.

Fontaine, F.R., Dunlop, R.A., Petersen, D.R., and Burcham, P.C. (2002) Oxidative bioactivation of crotyl alcohol to the toxic endogenous aldehyde crotonaldehyde: association of protein carbonylation with toxicity in isolated mouse hepatocytes. Chemical Research in Toxicology 15, 1051-1058.

Burcham, PC, Kaminskas, LM, Fontaine, FR, Petersen, DR, & Pyke, SM (2002) Aldehyde-sequestering drugs: tools for studying protein damage by lipid peroxidation products. Toxicology, 181-182C, 229-236.

Ghaoui, R., Sallustio, B.C., Burcham, PC and Fontaine, F.R. (2003) UDP-Glucuronosyltransferase-dependent bioactivation of clofibric acid to a DNA-damaging intermediate in mouse hepatocytes. Chemico-Biological Interactions 145, 201-211.

Froscio, S.M., Humpage, A.R., Burcham, P.C., and Falconer, I.R. (2003) Cylindrospermopsin-induced protein synthesis inhibition and its dissociation from acute toxicity in mouse hepatocytes. Environmental Toxicology, 18, 243-251.

Burcham, P. C., Fontaine, F. R., Petersen, D. R., and Pyke, S. M. (2003) Reactivity with tris(hydroxymethyl)aminomethane confounds immunodetection of acrolein-adducted proteins. Chemical Research in Toxicology, 16, 1196-1201.

Burcham, P.C., Fontaine, F.R., Kaminskas, L.M., Petersen, D.R. and Pyke, S.M. (2004) Protein adduct-trapping by hydrazinophthalazine drugs: Role in cytoprotection against acrolein-mediated toxicity. Molecular Pharmacology, 65, 655-664.

Frank R. Fontaine, Yvette DeGraaf, Roula Ghaoui, Benedetta C. Sallustio, Edwards J., and Burcham, P. C. (2004). Optimisation of the comet genotoxicity assay in freshly isolated murine hepatocytes: detection of strong in vitro DNA-damaging properties for styrene. Toxicology In Vitro, 18, 343-350

Kaminskas, L. M., Pyke, S. M., and Burcham, P. C. (2004) Strong protein adduct-trapping accompanies abolition of acrolein-mediated hepatotoxicity by hydralazine in mice. Journal of Pharmacology & Experimental Therapeutics 310, 1003-1010.

Kaminskas, L. M., Pyke, S. M., and Burcham, P. C. (2004) Hydrazinophthalazine drugs efficiently trap the toxic short-chain 2-alkenals acrolein and crotonaldehyde. Organic & Biomolecular Chemistry, 2, 2578 - 2584.

Greenrod W, Stockley CS, Burcham P, Abbey M and Fenech M (2005) Moderate intake of de-alcoholised red wine, but not alcohol, is protective against radiation-induced DNA damage ex vivo – results of a comparative in vivo intervention study in younger men. Mutation Research (in press).