Centre for Microscopy, Characterisation & Analysis

(formerly Biomedical Imaging and Analysis Facility)

The University of Western Australia

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The following is a list of some of the equipment located in BIAF. More details will be available as this list is updated.

MAJOR EQUIPMENT

Biorad MRC 1000/1024 UV Laser Scanning Confocal Microscope on Nikon Diaphot 300

Biorad DVC 250 Line Scanning Confocal Microscope

Becton Dickinson FACS Vantage Cell Sorter. Three laser lines (351nm, 488nm & 647nm), 5 fluorescence parameters, 2 populations simultaneously, up to 5,000 events per second.

Becton Dickinson FACS Canto II Flow Cytometer with High Throughput Sampler. - equipped with two lasers (488nm & 633nm), this instrument supports enhanced 6-colour fluorescence analysis plus forward- and side-scatter measurements at an acquisition rate of up to 10,000 events per second. The HTS will run 96 and 384 well plates with a sample analysis time of approximately 15 minutes per 96 well plate and a sample volume as low as 50ul.

Becton Dickinson FACS Calibur Flow Cytometer. - equipped with two lasers (488nm & 635nm), this instrument supports routine 4-colour analysis (FITC, PE, PerCP/PE-Cy5/PI & APC) plus forward- and side-scatter measurements.

Becton Dickinson FACScan Flow Cytometer. - equipped with a single laser (488nm), this instrument supports routine 3-colour analysis (FITC, PE, & PerCP/PE-Cy5/PI), plus forward- and side-scatter measurements.

PALM Laser Microdissector with Laser Tweezers

Perkin Elmer CS1000 Autoplex Analyzer (Luminex 200 Bead Array System) - able to simultaneously measure up to 100 analytes in a single sample with a volume as low as 25ul.

LASERS

Coherent Enterprise II 622 Argon UV 250mW (351nm, 363.5nm, 488nm, 514.5nm)

Coherent Enterprise II 621 Argon UV 250mW (351nm, 488nm)

Coherent Innova 70 Spectrum Krypton/Argon 2.5W (457.9nm, 488nm, 514.5nm, 520.8nm, 530.9nm, 568.2nm, 647.1nm)

MellesGriot HeNe (green) 1.5mW (543nm)

MellesGriot HeNe (red) 7mW (633nm)

ALC 100mW multiline Argon laser (457.9nm, 476nm, 488nm, 49??nm, ???, ???, 514.5nm)

Spectra-Physics 15mW Argon laser (488nm)

MellesGriot 25mW solid state yellow (561nm)

ACCESSORY EQUIPMENT

Olympus IMT-2 inverted microscope with epifluorescence

Olympus BH-2 upright microscope

Olympus DP-11 digital camera

Photonic Sciences CV12/HI cooled CCD camera

Heated stages for Olympus IMT-2 and Nikon Diaphot 300

Vibratome

MICROSCOPE OBJECTIVES

A full list of all of the objectives and their characteristics can be found by downloading the "Microscope Objectives" pdf file (108KB).

COMPUTING WORKSTATIONS AND SOFTWARE

Pentium 4 / 2.8GHz, 2GB RAM, Windows XP PC

Pentium 4, dual core 3GHz, 4GB RAM, Windows XP 64 bit PC

Flow cytometry analysis workstations

Biorad COMOS and Lasersharp workstations

Image Pro Plus version 4.5 image analysis software

Image J image analysis software

Adobe Photoshop versions 5, 6, 7, CS and CS2

Bitplane Imaris 3D visualisation software

OTHER COMPUTING/IMAGING EQUIPMENT

Nikon E8000 film scanner

Agfa Fotocolor 35mm slide maker

Codonics NP1600 dye-sublimation printer

Nikon D70S digital camera